Journal: Diagnostic Pathology

Article Title: The ectopic expression of BRCA1 is associated with genesis, progression, and prognosis of breast cancer in young patients

doi: 10.1186/1746-1596-7-181

Figure Lengend Snippet: A (young group) BRCA1 protein immunohistochemical staining was positive. The positive products localized in the cytoplasm (× 400). B (young group) BRCA1 protein immunohistochemical staining was positive. The positive products localized in the nucleus (× 400). C (old group) BRCA1 protein immunohistochemical staining was positive. The positive products localized in the cytoplasm (× 400). D (young group) WWOX protein immunohistochemical staining was positive. The positive products localized in the cytoplasm (× 400). E (old group) WWOX protein immunohistochemical staining was positive. The positive products localized in the cytoplasm (× 400) . F (young group) ER protein immunohistochemical staining was positive. The positive product positioning in the nucleus (× 400). G (young group) positive immunohistochemical staining of PR proteins. The positive products localized in the nucleus (× 400) H (young group) C-erbB2 protein immunohistochemical staining was positive. The positive products localized in the membrane (× 400). I (young group) Ki67 protein immunohistochemical staining was positive. The positive products localized in the nucleus (× 400).

Article Snippet: The WWOX antibody was purchased from Beijing Boaosen Biotechnology Co., Ltd. BRCA1 antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. ER, PR, and Ki67 were expressed in the nucleus.

Techniques: Immunohistochemical staining, Staining, Membrane

Journal: Diagnostic Pathology

Article Title: The ectopic expression of BRCA1 is associated with genesis, progression, and prognosis of breast cancer in young patients

doi: 10.1186/1746-1596-7-181

Figure Lengend Snippet: Analysis of the correlation between the BRCA1 results and clinicopathological parameters in the young groups and old groups

Article Snippet: The WWOX antibody was purchased from Beijing Boaosen Biotechnology Co., Ltd. BRCA1 antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. ER, PR, and Ki67 were expressed in the nucleus.

Techniques:

Journal: Diagnostic Pathology

Article Title: The ectopic expression of BRCA1 is associated with genesis, progression, and prognosis of breast cancer in young patients

doi: 10.1186/1746-1596-7-181

Figure Lengend Snippet: A The PCR product of exon 2 of the BRCA1 gene (259 bp). B The PCR product of exon 20 of the BRCA1 gene (401 bp).

Article Snippet: The WWOX antibody was purchased from Beijing Boaosen Biotechnology Co., Ltd. BRCA1 antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. ER, PR, and Ki67 were expressed in the nucleus.

Techniques:

Journal: Diagnostic Pathology

Article Title: The ectopic expression of BRCA1 is associated with genesis, progression, and prognosis of breast cancer in young patients

doi: 10.1186/1746-1596-7-181

Figure Lengend Snippet: A The partial DNA sequencing results of exon 2 of BRCA1 gene of patient 6. B The partial DNA sequencing results of exon 20 of BRCA1 gene of patient 6.

Article Snippet: The WWOX antibody was purchased from Beijing Boaosen Biotechnology Co., Ltd. BRCA1 antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. ER, PR, and Ki67 were expressed in the nucleus.

Techniques: DNA Sequencing

Journal: Diagnostic Pathology

Article Title: The ectopic expression of BRCA1 is associated with genesis, progression, and prognosis of breast cancer in young patients

doi: 10.1186/1746-1596-7-181

Figure Lengend Snippet: Comparative analysis of the immunohistochemical results of young and old patient groups

Article Snippet: The WWOX antibody was purchased from Beijing Boaosen Biotechnology Co., Ltd. BRCA1 antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. ER, PR, and Ki67 were expressed in the nucleus.

Techniques: Immunohistochemical staining

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Cross-analysis of the methylation of RAD51 and BRCA1 genes in tumor and non-tumor mucosa, considering the 37 cases with paired tumoral and non-tumoral samples.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Methylation

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: RAD51 , ATR , BRCA1 , BRCA2 , and ATM mRNA expression in gastric adenocarcinoma and non-tumor gastric mucosa samples.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Expressing

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Comparison of RAD51 , ATR , BRCA1 , BRCA2 , and ATM mRNA expression in tumor and non-tumor mucosa, considering the paired samples of the same individuals. The results are presented in box plots, using a logarithmic scale (+1 was added as a constant to allow the logarithmic representation of null values).

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Comparison, Expressing

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Comparison between RAD51 , ATR , BRCA1 , BRCA2 , and ATM mRNA expression in patients with previous neoadjuvant chemotherapy and in those without neoadjuvant chemotherapy, considering tumor samples and non-tumor mucosa samples.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Comparison, Expressing

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Association between immunohistochemistry and mRNA levels in tumor samples.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Immunohistochemistry

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Correlation between RAD51 , ATR , BRCA1 , BRCA2 , and ATM mRNA expression and histological type, histological grade, perineural invasion, and vascular invasion in tumor samples.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Expressing

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Correlation between RAD51 , ATR , BRCA1 , BRCA2 , and ATM mRNA expression and depth of invasion (pT), regional lymph node metastasis, distant metastasis, and stage in tumor samples.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Expressing

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Comparison between the mRNA levels in the tumors of patients who died and those who did not, considering the following groups: total population, excluding neoadjuvant therapy, with only surgical treatment, and with adjuvant or neoadjuvant treatment.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Comparison, Adjuvant

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Kaplan-Meier curves comparing the overall survival of the 24 patients with tumor mRNA expression above the median with that of the 24 below the median, for RAD51 , ATR , BRCA1 , BRCA2 , and ATM .

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Expressing

Journal: Nutrients

Article Title: Epigenetic Activation of BRCA1 by Genistein In Vivo and Triple Negative Breast Cancer Cells Linked to Antagonism toward Aryl Hydrocarbon Receptor

doi: 10.3390/nu11112559

Figure Lengend Snippet: Lifetime exposure to genistein (GEN) decreases basal Brca1 cytosine-guanine dinucleotide (CpG) methylation and aryl hydrocarbon receptor (AHR) activity in mouse offspring mammary tissue. Mice were exposed to diets containing 0, 4, or 10 ppm GEN starting at conception and through nursing, weaning, and adulthood. ( A ) Brca1 CpG methylation in a mammary gland of post-natal day 50 (PND50) female offspring. Bars represent the mean ratios of methylated Brca1 to unmethylated Brca1 amplicons ± SEM from ≥7 individual animals. ( B ) Cyp1b1 mRNA expression in PND50 mammary glands. Bars represent mean RQ ± SEM from ≥7 individual animals. Means with different letters (a > b > c) or asterisks (**, p < 0.01) indicate statistical significance ( p < 0.05).

Article Snippet: Immunoblotting was carried out with antibodies raised against BRCA1 (Boster Bio, Pleasanton, CA, USA; Ref. PB9015), ERα (Santa Cruz, Ref. MC-20), AHR (Santa Cruz Biotechnology Inc, Dallas, TX, USA; Ref. B-11), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Origene, Rockville, MD, USA; Ref. TA890003).

Techniques: CpG Methylation Assay, Activity Assay, Methylation, Expressing

Journal: Nutrients

Article Title: Epigenetic Activation of BRCA1 by Genistein In Vivo and Triple Negative Breast Cancer Cells Linked to Antagonism toward Aryl Hydrocarbon Receptor

doi: 10.3390/nu11112559

Figure Lengend Snippet: BRCA1 CpG hypermethylation associates with constitutively active AHR in triple negative breast cancers (TNBC) cells. ( A ) Representative Western blot images comparing immunocomplexes of AHR and internal standard glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in HCC38 and UACC3199 cells. ( B ) Data from methylation-specific PCR (MSP) comparing BRCA1 and ESR1 CpG methylation in HCC38 and MCF7 cells. ( C ) Bands represent immunocomplexes for BRCA1, estrogen receptor (ER)α, AHR, and internal standard GAPDH in MCF7 and HCC38 cells. ( D ) Comparison of AHR mRNA expression in HCC38 and MCF7 cells. ( E ) Expression of AHR targets CYP1A1 and CYP1B1 in HCC38 and MCF7 cells. ( F ) CYP1A1 and CYP1B1 expression in HCC38 cells treated with the AHR antagonist CH-223191. Bars represent sample means ± SEM from ≥3 biological replicates from individual experiments. VEH: vehicle-treated control. Asterisks indicate significant differences (*, p < 0.05; **, p < 0.01).

Article Snippet: Immunoblotting was carried out with antibodies raised against BRCA1 (Boster Bio, Pleasanton, CA, USA; Ref. PB9015), ERα (Santa Cruz, Ref. MC-20), AHR (Santa Cruz Biotechnology Inc, Dallas, TX, USA; Ref. B-11), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Origene, Rockville, MD, USA; Ref. TA890003).

Techniques: Western Blot, Methylation, CpG Methylation Assay, Comparison, Expressing, Control

Journal: Nutrients

Article Title: Epigenetic Activation of BRCA1 by Genistein In Vivo and Triple Negative Breast Cancer Cells Linked to Antagonism toward Aryl Hydrocarbon Receptor

doi: 10.3390/nu11112559

Figure Lengend Snippet: Genistein rescues BRCA1 protein levels in TNBC cells with CpG hypermethylated BRCA1 . Bands are immunocomplexes of BRCA1 and internal standard GAPDH in HCC38 cells treated with ( A ) GEN, ( B ) α-naphthoflavone (NF), ( C ) CH-223191, and ( D ) galangin (GAL). Images are representative blots from ≥3 individual experiments. For densitometry histograms, bars represent mean RQ ± SEM from ≥3 biological replicates from individual experiments. Asterisks indicate significant differences (*, p < 0.05; ***, p < 0.001).

Article Snippet: Immunoblotting was carried out with antibodies raised against BRCA1 (Boster Bio, Pleasanton, CA, USA; Ref. PB9015), ERα (Santa Cruz, Ref. MC-20), AHR (Santa Cruz Biotechnology Inc, Dallas, TX, USA; Ref. B-11), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Origene, Rockville, MD, USA; Ref. TA890003).

Techniques:

Journal: Nutrients

Article Title: Epigenetic Activation of BRCA1 by Genistein In Vivo and Triple Negative Breast Cancer Cells Linked to Antagonism toward Aryl Hydrocarbon Receptor

doi: 10.3390/nu11112559

Figure Lengend Snippet: Rescue of BRCA1 expression is linked to CpG demethylation in TNBC cells. The average ratio of mBRCA1 / umBRCA1 in HCC38 cells treated for 96 h with ( A ) GEN or ( B ) GAL. Bars represent sample means ± SEM from ≥3 biological replicates from individual experiments. Asterisks denote significance (*, p < 0.05).

Article Snippet: Immunoblotting was carried out with antibodies raised against BRCA1 (Boster Bio, Pleasanton, CA, USA; Ref. PB9015), ERα (Santa Cruz, Ref. MC-20), AHR (Santa Cruz Biotechnology Inc, Dallas, TX, USA; Ref. B-11), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Origene, Rockville, MD, USA; Ref. TA890003).

Techniques: Expressing

Journal: Nutrients

Article Title: Epigenetic Activation of BRCA1 by Genistein In Vivo and Triple Negative Breast Cancer Cells Linked to Antagonism toward Aryl Hydrocarbon Receptor

doi: 10.3390/nu11112559

Figure Lengend Snippet: Antagonism toward AHR binding at the BRCA1 promoter by GEN. Results show the relative binding of AHR at the BRCA1 promoter from chromatin immunoprecipitation (ChIP) assays in ( A ) HCC38 cells treated with GEN and various AHR antagonists; and ( B ) MCF7 cells treated with estradiol (E2) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the presence or absence of GEN and GAL. Relative binding was determined as the percent input (ChIP/Input*100) from treated samples normalized to VEH ( A ) or E2 ( B ) controls. Bars represent sample means ± SEM from ≥3 biological replicates from individual experiments. Means with different letters (a > b) or asterisks (*) indicate significance (*, p < 0.05).

Article Snippet: Immunoblotting was carried out with antibodies raised against BRCA1 (Boster Bio, Pleasanton, CA, USA; Ref. PB9015), ERα (Santa Cruz, Ref. MC-20), AHR (Santa Cruz Biotechnology Inc, Dallas, TX, USA; Ref. B-11), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Origene, Rockville, MD, USA; Ref. TA890003).

Techniques: Binding Assay, Chromatin Immunoprecipitation